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On the next morning, dialysate samples have been collected at 20 min intervals, and dopamine and five-HT degrees were being quantitatively identified making use of microbore HPLC-ECD as described elsewhere (Baumann et al. 2011). Rats were randomly assigned to groups receiving possibly drug or saline injections. After 3 stable baseline samples had been received, rats gained two sequential intravenous (i.v.) injections of drug: just one dose at time zero, followed by a threefold larger dose 60 min later on. Regulate rats acquired sequential i.v. injections of saline (1 mL/kg) in accordance with the exact same agenda. Microdialysis samples have been collected every 20 min all over the submit-injection duration of a hundred and twenty min. Ambulation inside the arena, described as movement in the horizontal aircraft, was quantified in twenty min bins that coincided with assortment of dialysate samples. At the conclusion of the experiments, rats had been euthanized with CO2 and decapitated. The brain sections ended up examined to validate placement of microdialysis probe suggestions within the nucleus accumbens. Only All those rats with right placements have been A part of info analyses.
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